A novel technique for identification of Ascaris suum cohorts in pigs     

Nejsum P  Thamsborg SM  Jørgensen C  Fredholm M  Roepstorff A 《Veterinary parasitology》2008,154(1-2):171-174

The objective of the present study was to develop a fast, cheap and reliable technique for identifying different cohorts of the swine parasite, Ascaris suum. A polymerase chain reaction linked restriction fragment length polymorphism (PCR-RFLP) technique on mt-DNA was used to identify unique haplotypes of four gravid A. suum females on agarose gels after eggs were recovered from each of the worms. Each of four pigs was inoculated with 2000 embryonated eggs originating from one of the four identified Ascaris haplotypes, respectively. Ascaris larvae were isolated from the small intestine at day 14 post-infection using an agar technique. Single larvae from each pig were transferred to 96-well PCR plates and a simple DNA extraction using a worm lysis buffer was carried out and followed by the PCR-RFLP analysis. More than 100 larvae from each of the four pigs were analysed and all were found to have the same haplotype as the parental female. We conclude that unique haplotypes of female A. suum and offspring can be identified by means of PCR-RFLP on mt-DNA and suggest that this method can be used in future research on Ascaris population biology using cohorts with distinct mt-DNA profile.  相似文献   

Ultrasonographic description of tendons and ligaments at the palmar (plantar) aspect of the distal limb in the one humped camel (Camelus dromedarius)     

SM Gadallah  AM Sharshar  SA Elsharkawy  MS Fadel 《Australian veterinary journal》2023,101(10):397-408

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Femoral component failure in canine cemented total hip replacement: a report of two cases     

GD Yates  SM Wasik  GA Edwards 《Australian veterinary journal》2010,88(6):225-230

Femoral stem fracture is reported as an uncommon late complication of cemented total hip replacement in two dogs. In each case surgical salvage was achieved by extirpation of the proximal unstable component of the femoral stem, resulting in acceptable limb function. To the authors' knowledge, intramedullary femoral stem failure has not been previously reported after cemented total hip replacement in the dog. Factors believed to have contributed to implant failure in these dogs are discussed and compared with the same complication in humans.  相似文献   

Genetic analysis of Trichuris suis and Trichuris trichiura recovered from humans and pigs in a sympatric setting in Uganda     

Nissen S  Al-Jubury A  Hansen TV  Olsen A  Christensen H  Thamsborg SM  Nejsum P 《Veterinary parasitology》2012,188(1-2):68-77

The whipworms Trichuris trichiura and Trichuris suis in humans and pigs, respectively, are believed to be two different species yet closely related. Morphologically, adult worms, eggs and larvae of the two species are indistinguishable. The aim of this study was to examine the genetic variation of Trichuris sp. mainly recovered from natural infected pigs and humans. Worm material isolated from humans and pigs living in the same geographical region in Uganda were analyzed by PCR, cloning and sequencing. Measurements of morphometric characters were also performed. The analysis of the ITS-2 (internal transcribed spacer) region showed a high genetic variation in the human-derived worms with two sequence types, designated type 1 and type 2, differing with up to 45%, the type 2 being identical to the sequence found in pig-derived worms. A single human-derived worm showed exclusively the type 2-genotype (T. suis-type) and three cases of 'heterozygote' worms in humans were identified. However, the analysis showed that sympatric Trichuris primarily assorted with host origin. Sequence analysis of a part of the genetically conserved β-tubulin gene confirmed two separate populations/species but also showed that the 'heterozygote' worms had a T. suis-like β-tubulin gene. A PCR-RFLP on the ITS-2 region was developed, that could distinguish between worms of the pig, human and 'heterozygote' type. The data suggest that Trichuris in pigs and humans belong to two different populations (i.e. are two different species). However, the data presented also suggest that cross-infections of humans with T. suis takes place. Further studies on sympatric Trichuris populations are highly warranted in order to explore transmission dynamics and unravel the zoonotic potential of T. suis.  相似文献   

Use of Artemisia annua as a natural coccidiostat in free-range broilers and its effects on infection dynamics and performance     

de Almeida GF  Horsted K  Thamsborg SM  Kyvsgaard NC  Ferreira JF  Hermansen JE 《Veterinary parasitology》2012,186(3-4):178-187

This work investigated the preventive effect of Artemisia annua L. dried leaves supplied as a botanical coccidiostat to two broiler genotypes reared in a Danish free-range system in a factorial experiment (two genotypes and ± supplement of dried A. annua leaves). The genotypes White Bresse L40, a pure slow-growing line, and Kosmos 8 Ross, a hybrid genotype with medium growing characteristics, were used. Broilers were raised indoor until 29-days-old and kept free of parasites. Twelve groups of 30 randomly selected broilers were placed in the range forming three replicates for each treatment combination. The paddocks were cultivated with a mix of grass and clover. A separate group of broilers was naturally infected with Eimeria spp. oocysts and five animals nominated as "seeders" were introduced to the above mentioned 12 groups, 10 days after its formation, with each group consisting of 35 animals per plot. This infection strategy was meant to imitate the transmission pathway observed at farm level. Ten individual birds from each of the 12 groups, in total 120 animals of mixed sex, were monitored twice weekly for 30 days for oocysts excretion. PCR of pooled faecal samples, oocyst morphology and localization upon necropsy were used to identify the Eimeria species involved in the infection. In general, broilers from both genotypes in the range coped well with a coccidia infection caused by Eimeria acervulina and Eimeria maxima as no clinical symptoms, or deaths, were reported during the experiment. In general, broilers supplemented with A. annua dried leaves showed a significantly (p<0.05) reduced number of excreted oocysts during the infection with no interaction to genotype. Females generally had a significantly higher shedding of oocysts than males (p<0.05). The overall body weight gain and the daily weight gain when infection was subdued showed a three-way interaction among genotype, sex and treatment - accounted mainly for the fact that Kosmos females responded positively to the Artemisia treatment while Kosmos males responded negatively, and only minor differences were found between sexes for the White Bresse genotype. In conclusion, supply of A. annua dried leaves as a botanical coccidiostat significantly reduced oocyst output in free ranged broilers and thus may form part of a strategy to prevent commercial losses.  相似文献   

Condensed tannins act against cattle nematodes     

Novobilský A  Mueller-Harvey I  Thamsborg SM 《Veterinary parasitology》2011,182(2-4):213-220

The use of natural plant anthelmintics was suggested as a possible alternative control of gastrointestinal nematodes (GIN) in ruminants. Direct anthelmintic effects of tannin-containing plants have already been shown in sheep and goat GIN. These anthelmintic properties are mainly associated with condensed tannins. In the present study, we evaluated possible in vitro effects of three tannin-containing plants against bovine GIN. Effects of Onobrychis viciifolia, Lotus pedunculatus and Lotus corniculatus condensed tannin (CT) extracts on Cooperia oncophora and Ostertagia ostertagi were determined by a larval feeding inhibition assay (LFIA) and a larval exsheathment assay (LEA). In the LFIA, all three plant extracts significantly inhibited larval feeding behaviour of both C. oncophora and O. ostertagi first stage larvae in a dose-dependent manner. The L. pedunculatus extract, based on EC(50) (effective concentration for 50% inhibition), was the most effective against both nematodes, followed by O. viciifolia and L. corniculatus. The effect of CT extracts upon larval feeding behaviour correlates with CT content and procyanidin/prodelphidin ratio. Larval exsheathment of C. oncophora and O. ostertagi L3 larvae (third stage larvae) was also affected by CT extracts from all three plants. In both in vitro assays, extracts with added polyvinylpolypyrrolidone, an inhibitor of tannins, generated almost the same values as the negative control; this confirms the role of CT in the anthelmintic effect of these plant extracts. Our results, therefore, indicated that tannin-containing plants could act against cattle nematodes.  相似文献   

Serological responses of Australian horses using a commercial duplex indirect ELISA following vaccination against strangles     

CM El‐Hage  H Bannai  AK Wiethoelter  SM Firestone  CM Heislers  JL Allen  AS Waller  JR Gilkerson 《Australian veterinary journal》2019,97(7):220-224

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Prevalence of foot lesions in Danish Holstein cows     

Capion N  Thamsborg SM  Enevoldsen C 《The Veterinary record》2008,163(3):80-85

In a cross-sectional study of 6240 Danish Holstein cows in 55 herds using loose-housing systems, sole haemorrhages and heel horn erosions occurred frequently in almost all the herds. Digital dermatitis occurred in 47 of the 55 herds (85 per cent). Spearman correlation coefficients revealed relationships between lameness and sole ulcer (0.36), between heel horn erosion and sole haemorrhage (0.39), between heel horn erosion and interdigital dermatitis (0.29) and between sole ulcer and double sole (0.26). The prevalence of heel horn erosion, sole haemorrhage, interdigital dermatitis and digital dermatitis appeared to be most affected by herd-level factors. The associations between individual foot trimmers and all the foot lesions were statistically significant. The risk of interdigital dermatitis increased with decreasing amounts of bedding. Cows housed throughout the year had a marginally higher risk of sole haemorrhage.  相似文献   

Automated identification of intensive animal production locations from aerial photography          下载免费PDF全文

KJ Sheffield  JC Hunnam  TN Cuzner  EM Morse‐McNabb  SM Sloan  J Nunan  J Smith  W Harvey  H Lewis 《Australian veterinary journal》2018,96(9):323-331

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In Vitro Maturation and Fertilization of Buffalo Oocytes: Effects of Storage of Ovaries, IVM Temperatures, Storage of Processed Sperm and Fertilization Media     

BM Ravindranatha  S Nandi  HM Raghu  SM Reddy 《Reproduction in domestic animals》2003,38(1):21-26

Studies were conducted to examine the possibility of preserving slaughterhouse‐derived buffalo ovaries at 4°C for 0 (control), 12 and 24 h to maintain the developmental competence of the oocytes (experiment 1), to assess the effect of incubation temperature during oocyte maturation on rates of in vitro maturation (IVM) and in vitro fertilization (IVF) of buffalo oocytes and embryo development (experiment 2), and to examine the effect of storage at 25°C for 0 (control), 4 and 8 h of frozen–thawed buffalo sperm and BO and H‐TALP as sperm processing and fertilization media on cleavage and embryo development in vitro of buffalo oocytes (experiment 3) in order to optimize the IVF technology in buffalo. Results suggested that storage of ovaries at 4°C for 12 or 24 h significantly (p < 0.05) reduced the developmental potential of oocytes. Incubation temperatures during the IVM influenced the fertilization rate but had no significant effect on maturation and subsequent embryo development. The incubation temperature of 38.5°C during IVM was found to be optimum for embryo production in vitro. Storage of frozen–thawed sperm at 25°C for 8 h significantly (p < 0.05) decreased its ability to cleave the oocytes. Sperm processed in BO medium had significantly (p < 0.05) higher ability to cleave the oocytes than the H‐TALP medium.  相似文献